SIR Biology Investigation Abstract
ANTIBODY EFFECT ON SYNAPTIC STRUCTURE
Presenter:
Lynn Jiang, Illinois Mathematics and Science Academy
Advisor:
Dr. Jaime Grutzendler, Feinberg School of Medicine
Abstract:
Research over the years indicates that immune system attacks on the nervous system can often lead to detrimental health effects. In given diseases, immune mechanisms may be able to cross the blood brain barrier and target either glia or neurons. However, the complete effect of these attacks on the brain is not thoroughly known. To investigate this, we put specific emphasis on examining synaptic transmission, experimenting with effect of antibodies on synaptic structure and plasticity. Within a normal system, neurotransmitters are fired across the synapse as a communication device between neurons. By binding to the post-synaptic channels, neurotransmitters can open the channels, causing an influx of substances such as calcium. However, this communication is regulated by concentration levels so that the channels are not open for an over-extended period of time. Through direct injection into the cerebral spinal fluid around the brain, we replaced the neurotransmitters with antibodies, thus upsetting the regulation and potentially opening the channels indefinitely. Using secondary antibody fluorescence, we can determine the success of the injection and then proceed to observe structural change by imaging with two-photon microscopy. While in the preliminary stages, these observations will be useful in better comprehension of the effects of antibodies on synaptic structure.